Many long for a return to a post-pandemic "normal," which, for some, may entail concerts, travel, and large gatherings. But how to keep safe amid these potential public health risks?
One possibility, according to a study, is dogs. A proof-of-concept investigation suggests that specially trained detection dogs can sniff out COVID-19-positive samples with 96% accuracy.
"This is not a simple thing we're asking the dogs to do," says Cynthia Otto, senior author on the work and director of the University of Pennsylvania School of Veterinary Medicine Working Dog Centre. "Dogs have to be specific about detecting the odour of the infection, but they also have to generalise across the background odours of different people: men and women, adults and children, people of different ethnicities and geographies."
In this initial study, researchers found the dogs could do that, but training must proceed with great care and, ideally, with many samples. The findings are feeding into another investigation that Otto and colleagues have dubbed "the T-shirt study," in which dogs are being trained to discriminate between the odours of COVID-positive, -negative, and -vaccinated individuals based on the volatile organic compounds they leave on a T-shirt worn overnight.
"We are collecting many more samples in that study – hundreds or more – than we did in this first one, and are hopeful that will get the dogs closer to what they might encounter in a community setting," Otto says.
Through the Working Dog Centre, she and colleagues have had years of experience training medical-detection dogs, including those that can identify ovarian cancer. When the pandemic arrived, they leveraged that expertise to design a coronavirus detection study.
Collaborators Ian Frank from the Perelman School of Medicine and Audrey Odom John from the Children's Hospital of Philadelphia provided SARS-CoV-2-positive samples from adult and paediatric patients, as well as samples from patients who had tested negative to serve as experimental controls. Otto worked closely with coronavirus expert Susan Weiss of Penn Medicine to process some of the samples in Penn's Biosafety Level 2+ laboratory to inactivate the virus so they would be safe for the dogs to sniff.
Because of workplace shutdowns due to the pandemic, instead of working with dogs at Penn Vet, the researchers partnered with Pat Nolan, a trainer with a facility in Maryland.
Eight Labrador retrievers and a Belgian Malinois that had not done medical-detection work before were used in the study. First the researchers trained them to recognize a distinctive scent, a synthetic substance known as universal detection compound (UDC). They used a "scent wheel" in which each of 12 ports is loaded with a different sample and rewarded the dog when it responded to the port containing UDC.
When the dogs consistently responded to the UDC scent, the team began training them to respond to urine samples from SARS-CoV-2 positive patients and discern positive from negative samples. The negative samples were subjected to the same inactivation treatment — either heat inactivation or detergent inactivation – as the positive samples.
Processing the results with assistance from Penn criminologist and statistician Richard Berk, the team found that after three weeks of training all nine dogs were able to readily identify SARS-CoV-2 positive samples, with 96% accuracy on average. Their sensitivity, or ability to avoid false negatives, however, was lower, in part, the researchers believe, because of the stringent criteria of the study: If the dogs walked by a port containing a positive sample even once without responding, that was labelled a "miss."
The researchers ran into many complicating factors in their study, such as the tendency of the dogs to discriminate between the actual patients, rather than between their SARS-CoV-2 infection status. The dogs were also thrown off by a sample from a patient that tested negative for SARS-CoV-2 but who had recently recovered from COVID-19.
"The dogs kept responding to that sample, and we kept telling them no," Otto says. "But obviously there was still something in the patient's sample that the dogs were keying in on."
Major lessons learned from the study, besides confirming that there is a SARS-CoV-2 odour that dogs can detect, were that future training should entail large numbers of diverse samples and that dogs should not be trained repeatedly on the samples from any single individual.
"That's something we can carry forward not only in our COVID training but in our cancer work and any other medical detection efforts we do," says Otto. "We want to make sure that we have all the steps in place to ensure quality, reproducibility, validity, and safety for when we operationalize our dogs and have them start screening in community settings."
Discrimination of SARS-CoV-2 infected patient samples by detection dogs: A proof of concept study
Jennifer L Essler, Sarah A Kane, Pat Nolan, Elikplim H Akaho, Amalia Z Berna, Annemarie DeAngelo, Richard A Berk, Patricia Kaynaroglu, Victoria L Plymouth, Ian D Frank, Susan R Weiss, Audrey R Odom John, Cynthia M Otto
Published in PLOS One on 14 April 2021
While the world awaits a widely available COVID-19 vaccine, availability of testing is limited in many regions and can be further compounded by shortages of reagents, prolonged processing time and delayed results. One approach to rapid testing is to leverage the volatile organic compound (VOC) signature of SARS-CoV-2 infection. Detection dogs, a biological sensor of VOCs, were utilized to investigate whether SARS-CoV-2 positive urine and saliva patient samples had a unique odor signature. The virus was inactivated in all training samples with either detergent or heat treatment. Using detergent-inactivated urine samples, dogs were initially trained to find samples collected from hospitalized patients confirmed with SARS-CoV-2 infection, while ignoring samples collected from controls. Dogs were then tested on their ability to spontaneously recognize heat-treated urine samples as well as heat-treated saliva from hospitalized SARS-CoV-2 positive patients. Dogs successfully discriminated between infected and uninfected urine samples, regardless of the inactivation protocol, as well as heat-treated saliva samples. Generalization to novel samples was limited, particularly after intensive training with a restricted sample set. A unique odor associated with SARS-CoV-2 infection present in human urine as well as saliva, provides impetus for the development of odor-based screening, either by electronic, chemical, or biological sensing methods. The use of dogs for screening in an operational setting will require training with a large number of novel SARS-CoV-2 positive and confirmed negative samples.
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