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Cryptococcal fungi in public spaces in SA

Large populations of potentially deadly cryptococcal fungi have been found on woody debris collected from old trees in two public areas in the centre of Cape Town and the Northern Cape.

After tuberculosis, cryptococcal meningitis is the leading cause of death in HIV/Aids patients in sub-Saharan Africa. In 2016, South Africa launched the world's largest national screening programme to detect cryptococcal meningitis in patients living with HIV. People become infected when they inhale the airborne microscopic spores produced by pathogenic cryptococci occurring in the environment.

The fungi were found and identified by PhD student Jo-Marie Vreulink as part of her research in the department of microbiology at Stellenbosch University (SU). The findings of her research have now been published.

This is the first time that both Cryptococcus neoformans and Cryptococcus gattii have been found in such large numbers on trees in South Africa. To date, only two studies (one from 2009 and the other published in 2011) have reported the presence of these pathogens in the South African environment. C. neoformans causes a severe form of meningitis, mostly in individuals with a compromised immune system. Generally, healthy people's immune systems are able to ward off the infection. C. gattii, on the other hand, can lead to meningitis in healthy individuals.

But while pathogenic cryptococci have been thoroughly researched from a clinical perspective, there is very little information available about their ecology and how they interact with the environment. This type of information can aid in curbing their spread from trees (their host) to the general public.

Profssor Alf Botha, from SU's department of microbiology and Vreulink's study leader, says he has been searching for Cryptococcus in South Africa since 2003. Worldwide, entire research groups are focusing on finding these fungi in the environment.

His research group has been working closely with Professor Teun Boekhout from the Westerdijk Fungal Biodiversity Institute in The Netherlands to ensure that the collected cryptococci are identified and characterised according to the most modern taxonomic methods.

Vreulink says initially they were looking for pathogenic cryptococci in woody debris sampled from pristine areas in South Africa, but with very little success. In 2007, as it became more and more evident worldwide that these fungi are found in areas where there is a combination of pigeons, old trees and large numbers of people, she changed tack and started looking in public spaces.

To her surprise the very first samples collected from a public park in the centre of Cape Town, delivered more colonies than she could handle.

"It was late on a Friday afternoon and I was working alone. I decided to check on the petri dishes that I prepared from the samples collected in Cape Town. On most of the dishes brown colonies – typical of these cryptococcal pathogens – were growing. This was such a rare occasion that I started working immediately to transfer the colonies to new petri dishes for identification. I was scared to death that the colonies will be overgrown by other microorganisms if I left it over the weekend," she recalls.

As part of her MSc and later PhD studies, her research efforts have been focused on understanding the biology and ecology of the single-celled yeasts that make up these brown colonies. She compared their genetic makeup to that of pathogens isolated from patients in South Africa, as well as to that of pathogenic cryptococci found elsewhere. She also looked at their resistance to commonly used antifungals and how environmental factors affect their survival in trees.

While Vreulink only collected samples from the two public spaces, she believes these pathogens are everywhere: "Methods used to isolate these fungi are simply not sensitive enough."

But there is still a lot that needs to be understood: "For now, I'm focusing on the ecology of these yeasts. I want to understand the population dynamics, the genetics and how these it interacts with their environment. If we can understand how they survive out there, we can use this knowledge to better predict how they can survive in their human host.

"At the moment, my research is generating more questions than answers. But that makes it even more exciting!"

Abstract
Knowledge of the environmental prevalence of members of the Cryptococcus neoformans/Cryptococcus gattii species complex is important, since cryptococcal infection is acquired from the environment. We determined whether trees located in two South African recreational areas harboured pathogenic cryptococci and compared the isolates to clinical isolates obtained from Western Cape hospitals with molecular typing techniques. The majority of isolates originating from trees in a public park in Cape Town (PPCT) were C. gattii sensu stricto, followed by C. neoformans sensu stricto genotype AFLP1/VNI. The PPCT trees might be a source of infection, since all genotype AFLP1/VNI isolates from these trees and one clinical isolate belonged to the same sequence type (ST), i.e. ST23. Recombination and basidiospore production might be occurring in PPCT trees that contained C. gattii s.s. isolates belonging to both mating types. The presence of C. gattii s.s. in PPCT trees might therefore pose a risk to human health.

Authors
Jo-Marie Vreulink, Kantarawee Khayhan, Ferry Hagen, Angela Botes, Leandra Moller, Teun Boekhout, Hester Vismer, Alfred Botha

[link url="http://www.sun.ac.za/english/Lists/news/DispForm.aspx?ID=5331"]Stellenbosch University material[/link]
[link url="http://www.sciencedirect.com/science/article/pii/S1754504817301186?via%3Dihub"]Fungal Ecology abstract[/link]

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