Repetitive ‘headers’ and accidental head impacts in soccer lead to changes in blood patterns linked to brain signalling pathways and potential alterations, shows a Norwegian study of 89 professional players.
In this latest research, experts analysed blood samples from 89 professional soccer players in a range of scenarios, including match-play and training, which involved heading. They also took samples generated by other high-intensity exercise aside from heading.
Results, published in the peer-reviewed journal Brain Injury, demonstrate “specific alterations” in the levels of the brain’s microRNAs, which are being recognised as blood biomarkers, associated with signalling pathways suggestive of brain alterations.
Looking at accidental head impacts specifically, the experts found the microRNAs (biomarkers) affected links to several signalling pathways involved in brain activity.
When assessing heading, the experts detected deregulation of other microRNAs – linked to yet another molecular signalling pathway. Importantly, these microRNAs were found to be unaffected by other high-intensity exercise.
Reporting on the findings, Stian Bahr Sandmo, of the Oslo Sports Trauma Research Center at the Norwegian School of Sport Sciences, who led an international team of experts, says the research adds further knowledge to what we understand about head impacts in soccer.
“This is a relatively small sample-size exploratory study,” he said, “but future findings expanding upon our research could ultimately lead to an improved understanding of the potential hazardous effects of repetitive head impacts. With millions of people playing soccer worldwide, this might ultimately have a substantial influence on public health.”
MicroRNAs are small molecules found in our cells that also circulate in different body fluids, like the blood (then referred to as “circulating microRNAs”). They help regulate gene expression, the process by which the instructions in our DNA are converted into a functional product, such as a protein.
They are involved in many different physiological processes. Previous studies have suggested certain microRNAs in the blood are altered in response to mild traumatic brain injury (TBI). This has led to new research, such as this current study, to explore how, and if, microRNAs can be used as brain injury biomarkers. Until now, the effects of soccer-related head impacts on microRNAs have been largely unexplored.
In this latest study, the team of multidisciplinary specialists wanted to uncover whether these head impacts change the levels of microRNAs –therefore reflecting short-term effects in the brain. The results were then compared with other high-intensity exercises. Depending on the findings, this would further add or detract to the growing evidence for microRNAs to be used as biomarkers for brain injury.
Blood samples were collected from 89 professional soccer players at rest. The players were aged 18-35 and were actively playing in the Eliteserien (the Norwegian premier league). Repeat samples were drawn after one hour, and again at 12 hours, after three conditions:
• accidental head impacts in a match (defined as any situation where a player appeared to be hit in the head, face or neck, the match was interrupted by the referee and the player remained lying on the ground for more than 15 seconds)
• repetitive headers during training
high-intensity exercise (as the effects of rigorous physical activity alone had to be accounted for, to isolate microRNAs specific to the brain injury and not just those affected by exercise).
The identified microRNAs were then validated to determine consistently deregulated microRNAs. The researchers also used bioinformatics to identify genes targeted by the microRNAs to characterise their roles in biological processes and signalling pathways.
When looking at accidental head injuries, their findings show the deregulation of eight microRNAs unaffected by high-intensity exercise: the target genes identified were linked to 12 signalling pathways, including the Wnt pathway, which, research suggests, may promote nerve cell regeneration after TBI. This also included Hedgehog signalling, which has been found (in animal studies) to reduce nerve cell death and damage after TBI.
Repetitive headers were found to alter the levels of six microRNAs and were associated with TGF-β signalling. Previous research has reported elevated TGF-βlevels in the fluid surrounding the brain after TBI, suggesting it might play a role in anti-inflammatory and neuroprotective signalling.
“Overall,” the team said, “the findings add further evidence to demonstrate how microRNAs may be used as brain injury biomarkers. These have the potential to differentiate injury severity and to discriminate between types of head impacts seen in soccer, if reproduced in further studies.”
Limitations of this current research include a relatively small sample size and that head-impact exposure was characterised using direct observation and video analysis, meaning the team was not able to quantify the biomechanical magnitudes of the impacts in the different conditions.
Study details
Changes in circulating microRNAs following head impacts in soccer
Stian Bahr Sandmo, Katarina Matyasova, Peter Filipcik, Martin Cente, Inga Katharina Koerte, Ofer Pasternak, Thor Einar Andersen, Truls Martin Straume-Næsheim, Roald Bahr, Igor Jurisica.
Published in Brain Injury on 16 February 2022
Abstract
Aim
To explore the short-term effects of accidental head impacts and repetitive headers on circulating microRNAs, accounting for the effects of high-intensity exercise alone.
Methods
Blood samples were collected from professional soccer players at rest. Repeat samples were drawn 1 h and 12 h after three conditions: (1) accidental head impacts in a match, (2) repetitive headers during training, and (3) high-intensity exercise. 89 samples were screened to detect microRNAs expressed after each exposure. Identified microRNAs were then validated in 98 samples to determine consistently deregulated microRNAs. Deregulated microRNAs were further explored using bioinformatics to identify target genes and characterise their involvement in biological pathways.
Results
Accidental head impacts led to deregulation of eight microRNAs that were unaffected by high-intensity exercise; target genes were linked to 12 specific signalling pathways, primarily regulating chromatin organisation, Hedgehog and Wnt signalling. Repetitive headers led to deregulation of six microRNAs that were unaffected by high-intensity exercise; target genes were linked to one specific signalling pathway (TGF-β). High-intensity exercise led to deregulation of seven microRNAs; target genes were linked to 31 specific signalling pathways.
Conclusion
We identified microRNAs specific to accidental head impacts and repetitive headers in soccer, potentially being useful as brain injury biomarkers.
See more from MedicalBrief archives:
Purposely heading a soccer ball linked with CNS symptoms
UK parliamentary inquiry into link between sport and long-term brain injury
Ball heading in soccer found worse than unintentional head impacts
Soccer: Defenders and outfielders have up to 5x greater dementia risk